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RICE SCIENCE  2010, Vol. 17 Issue (1): 35-42     DOI: 10.1016/S1672-6308(08)60102-X
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Diversity Analysis in Selected Non-basmati Scented Rice Collection
Sarika MATHURE1, 2, Narendra JAWALI2, Altafhusain NADAF1
1)Department of Botany, University of Pune, Pune-411007, India; 2)Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai-400085, India
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Abstract Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions, 5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) marker systems. The varieties analyzed by 11 RAPD and 8 ISSR primers yielded an average of 65% and 80% polymorphism, respectively. The average number of polymorphic bands generated per RAPD primer was 6 and per ISSR primer was 5.87. RAPD and ISSR data analysis individually could not segregate basmati and non-basmati scented rice accessions. However, the analysis using a combined data could group basmati and non-basmati scented rice accessions separately. The bands present specifically among three accessions of non-basmati scented rice were also identified. The study revealed a high genetic diversity among non-basmati scented rice accessions.
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Narendra JAWALI
Altafhusain NADAF
Key wordsrice   random amplified polymorphic DNA   inter-simple sequence repeat   diversity   landrace   diversity analysis   scented rice     
Received: 2009-10-04; Published: 2010-03-28

the University of Pune-Bhabha Atomic Research Centre collaborative research programme, financial assistance by Department of Science and Technology (DST), New Delhi and University of Pune, Pune India.

Corresponding Authors: Altafhusain NADAF   
 E-mail: abnadaf@unipune.ernet.in
Cite this article:   
Sarika MATHURE, Narendra JAWALI, Altafhusain NADAF. Diversity Analysis in Selected Non-basmati Scented Rice Collection[J]. RICE SCIENCE, 2010, 17(1): 35-42 .
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