RICE SCIENCE ›› 2010, Vol. 17 ›› Issue (1): 35-42 .DOI: 10.1016/S1672-6308(08)60102-X

• Research Paper • Previous Articles     Next Articles

Diversity Analysis in Selected Non-basmati Scented Rice Collection

Sarika MATHURE1, 2, Narendra JAWALI2, Altafhusain NADAF1   

  1. 1)Department of Botany, University of Pune, Pune-411007, India; 2)Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai-400085, India
  • Received:2009-10-04 Online:2010-03-28 Published:2010-03-28
  • Contact: Altafhusain NADAF
  • Supported by:

    the University of Pune-Bhabha Atomic Research Centre collaborative research programme, financial assistance by Department of Science and Technology (DST), New Delhi and University of Pune, Pune India.

Abstract: Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions, 5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) marker systems. The varieties analyzed by 11 RAPD and 8 ISSR primers yielded an average of 65% and 80% polymorphism, respectively. The average number of polymorphic bands generated per RAPD primer was 6 and per ISSR primer was 5.87. RAPD and ISSR data analysis individually could not segregate basmati and non-basmati scented rice accessions. However, the analysis using a combined data could group basmati and non-basmati scented rice accessions separately. The bands present specifically among three accessions of non-basmati scented rice were also identified. The study revealed a high genetic diversity among non-basmati scented rice accessions.

Key words: rice, random amplified polymorphic DNA, inter-simple sequence repeat, diversity, landrace, diversity analysis, scented rice