Biodiversity and Dynamics of Planthoppers and Their Natural Enemies 
in Rice Fields with Different Nitrogen Regimes

Abstract

Abstract: A field experiment was conducted to study the effect of different nitrogen fertilizer rates i.e. 200, 100 and 0 kg N/ha in
paddy fields at International Rice Research Institute, Manila, Philippines. Biodiversity of arthropods sampled by Blower-Vac, and
dynamics of planthoppers, egg parasitoids of Homoptera trapped by rice plants with eggs of brown planthoppers (BPH) Nilaparvata
lugens (Stål), and web spiders on rice canopy collected by sweeping net, were analyzed at different rice growth stages. The most
abundant arthropods were sampled at the milking stage of rice, totalling 116 species identified into 14 insect orders and 15 species
of spider in all samples. Meanwhile the number of arthropod species significantly increased with rice growth and the diversity
indices increased with the increase of nitrogen rate at the booting stage. On the other hand, in the dominant predators, Pardosa
pseudoannulata, Callitrichi formosana, Micraspis sp., Cyrtorhinus lividipennis, Veliidae sp. and Mesoveliidae sp., only C.
lividipennis and Micraspis sp. were increased significantly in abundance following the application of nitrogen at the milking stage of
rice. The egg parasitoids of plant-hoppers were predominated by Anagrus flaveolus and Oligosita sp. and their densities in the field
without nitrogen fertilizer were markedly higher than those in fields with 100 and 200 kg N/ha at both booting and milking stages of
rice. The number and web area of dominant residential spiders Tetragnatha sp. and Araneus sp. in rice canopy significantly
reduced with the increase of nitrogen fertilizer. The population density of planthoppers, included BPH and the white-backed
planthoppers (WBPH) Sogatella furcifera Horváth, peaked during the booting stage, however, the number of BPH in rice field with
200 kg N/ha was considerably higher than those in other two rice fields with 100 kg N/ha and 0 kg N/ha at the booting as well as
the milking stage. These results indicated that the rapid growth in populations of planthopper due to excessive nitrogen might be
attributed to the combination of reduction in control capacity of natural enemies and strong simulation of nitrogen to planthoppers.

Key words: Nilaparvata lugens, nitrogen fertilizer, biodiversity, natural enemy, rice field, biological control

LU Zhong-xian, S. VILLAREAL , YU Xiao-ping, K. L. HEONG , HU Cui. Biodiversity and Dynamics of Planthoppers and Their Natural Enemies
in Rice Fields with Different Nitrogen Regimes[J]. RICE SCIENCE, 2006, 13(3): 218-226 .

References

1 Mew T W, Borrmeo E, Hardy B. Exploiting biodiversity for sustainable pest management. Manila: International Rice Research Institute, 2001: 241.
2 Way M J, Heong K L. The role of biodiversity in the dynamics and management of insect pests of tropic irrigated rice: A view. Bull Entomol Res, 1994, 84: 567-587.
3 Zhu Y, Chen H, Fan J, Wang Y, Li Y, Chen J, Fan J X, Yang S, Hu L, Leung H, Mew T W, Teng P S, Wang Z, Mundt C C. Genetic diversity and disease control in rice. Nature, 2000,406: 718-722.
4 Way M J, Javier G. Approaches to a relevant understanding of biodiversity for tropical irrigated rice pest management. In: Exploiting Biodiversity for Sustainable Pest Management.Manila: IRRI, 2001: 3-14.
5 Simpson I C, Roger P A, Oficial R. Effects of nitrogen fertilizer and pesticide management on floodwater ecology in wetland rice field: II. Dynamics of microcrustaceans and dipteran larvae. Biol Fert Soils, 1994, 17: 138-146.
6 Settle W H, Ariawan H, Astuti E T, Cahyana W, Hakim A L, Hindayana D, Lestari A S. Managing tropical rice pests through conservation of generalist natural enemies and alterative prey. Ecology, 1996, 77: 1975-1988.
7 Driesche R G, Bellows T S. Biological Control. New York: Chapman & Hall, 1996.
8 Dyck V A, Thomas B. The brown planthopper problem. In: Brown Planthopper: Threat to Rice Production in Asia.Manila: IRRI, 1979: 3-20.
9 Hu J Z, Lu Q H, Yang J S, Yang L P, Gao N Y. Effects of fertilizer and irrigation on the population of main insect pests and the yield of rice. Acta Entomol Sin, 1986, 29(1): 49-54. (in Chinese with English abstract)
10 Ma K C, Lee S C. Occurrence of major rice insect pests at different transplanting times and fertilizer levels in paddy field. Korean J Appl Entomol, 1996, 35(2): 132-136.
11 Prasad J, Misra D S. Influence of different levels N, P and K on the population of leaffolder and planthoppers. In: ChelliahS, Balasubramanian M. Pest Management in Rice.Coimbatore, India: Tamil Nadu Agricultural University, 1983:245-254.
12 Wu L H, Zhu Z R. The relationship between rice leaf color and occurrence of rice diseases and insects and its mechanism. Chinese J Rice Sci, 1994, 8(4): 231-235. (in Chinese with English abstract)
13 Zhang G F, Lu C T, Shen X C, Wang W X. The synthesized ecological effect of rice density and nitrogen fertilizer on the occurrence of main rice pests. Acta Phytophyla Sin, 1995, 22(1): 38-44. (in Chinese with English abstract)
14 Schoenly K, Justo H D, Barrion A T, Harris M, Bottrell B G. Analysis of invertebrate biodiversity in a Philippine farmer’s irrigated rice field. Environ Entomol, 1998, 27: 1125-1136.
15 Bowdish T I, Stiling P. The influence of salt and nitrogen on herbivore abundance: Direct and indirect effects. Oecologia, 1998, 113: 400-405.
16 Siemann E, Tilman D, Haarstad J, Ritchie M. Experimental tests of the dependence of arthropod diversity on plant diversity. Am Nat, 1998, 152: 738-750.
17 Moran V C, Southwood T R E. The guild composition of arthropod communities in trees. J Animal Ecol, 1982, 51:289-306.
18 Haddad N M, Haarstad J, Tilman D. The effects of long-term nitrogen loading on grassland insect communities. Oecologia, 2000, 124: 73-84.
19 Symondson W O C, Sunderland K D, Greenstone M H. Can generalist predators be effective biocontrol agents? Ann Rev Entomol, 2002, 47: 561-594.
20 Clarige M F, Morgan J C, Steenkiste A E, Iman M, Damyanti D. Experimental field studies on predation and egg parasitism of rice brown planthopper in Indonesia. Agric & Forest Entomol, 2002, 4(3): 203-209.
21 Heong K L, Aquino G B, Barrion A T. Population dynamics of plant- and leafhoppers and their natural enemies in riceecosystems in the Philippines. Crop Prot, 1992, 11: 371-379.
22 Matteson P C. Insect pest management in tropic Asian irrigated rice. Ann Rev Entomol, 2000, 45: 549-574.
23 Preap V, Zalucki M P, Nesbitt H J, Jahn G C. Effectiveness of brown planthopper predators: Population suppression by two species of spiders, Pardosa pseudoannulata and Araneus inustus. J Asia-Pacific Entomol, 2001, 4(2): 187-193.
24 Schoenly K, Cohen J E, Heong K L, Litsinger J A, Aquino G B, Barrion A T, Arida G. Food web dynamics of irrigated rice fields at five elevations in Luzon, Philippines. Bull Entomol Res, 1996, 86: 451-466.
25 Sigagaard L. Early season natural biological control of insect pests in rice by spiders- and some factors in the management of the cropping system that may affect this control. In: Toft S, Scharff N. European Archnology 2000. Aarhus: Aarhus University Press, 2002: 57-64.
26 Sigagaard L, Toft S, Villareal S. Diet-dependent fecundity of the spiders Atypena formosana and Pardosa pseudoannulata, predators in irrigated rice. Agric & Forest Entomol, 2001, 3: 285-295.
27 Wu J C, Hu G W, Tang J, Shu Z L, Yang J S, Wan Z N, Ren Z C. Studies on the regulation effect of neutral insect on the community food web in paddy field. Acta Ecol Sin, 1994, 14(4): 381-386. (in Chinese with English abstract)
28 Guo Y J, Wang N Y, Jiang J W, Chen J W, Tang J. Ecological significance of neutral insects as nutrient bridge for predators in irrigated rice arthropod community. Chinese J Biol Control, 1995, 11(1): 5-9. (in Chinese with English abstract)
29 McNabb D M, Halaj J, Wise D H. Inferring trophic positions of generalist predators and their linkage to the detrital food web in agroecosystems: A stable isotope analysis. Pedobiologia, 2001, 45: 289-297.
30 Yasumatsu K, Wongsiri T, Tirawat J. The role of chironomidae in the rice ecosystem and the regulation of its population by biotic factors and chemicals. In: Yasumatasu K. Contributions to the Development of Integrated Rice Pest Control in Thailand. Tsukuba: Japan International Cooperation Agency, 1981: 156-171.
31 Yu X P, Heong K L, Hu C. Effect of various non-rice host on the growth, reproduction and predation of mired bug, Cyrtorhinus lividipennis Reuter. In: Rice IPM Conference –Integrating Science and People in Rice Pest management. Manila: Malaysian Agricultural Research Institute and International Rice Research Institute, 1998: 56-63.
32 Bernays E A, Chapman R F. Host-plant selection by phytophagous insects. New York: Chapman and Hall, 1994.
33 Zheng X S, Xu H X, Yu X P, Lu Z X, Chen J M. The selection of Anagrus sp. and its application in sticky board trap. Entomol J East China, 2001, 10: 126-180. (in Chinese with English abstract)
34 Shepard M B, Barrion A T, Litsinger J A. Friends of the rice farmer: Helpful insects, spiders, and pathogens. Manila: IRRI, 1987.
35 Lu Z X, Yu X P, Heong K L, Hu C. Effect of nitrogenous fertilization in rice fields on the predatory function of Cyrthorhinus lividipennis to Nilaparvata lugens St?l. Acta Entomol Sin, 2005, 48(1): 48-56. (in Chinese with English abstract)
36 Cohen J E, Schoenly K, Heong K L, Justo H, Arida G, Barrion A T, Litsinger J A. A food web approach to evaluating the effect of insecticide spraying on insect pest population dynamics in a Philippine irrigated rice ecosystem. J Appl Ecol, 1994, 31: 747-763.
37 Li R D, Ding J H, Wu G W, Shu D M. The Brown Planthopper and Its Population Management. Shanghai: Fudan University Press, 1996: 334. (in Chinese)
38 Ooi P A C. Ecology and surveillance of Nilaparvata lugens-implications for its management in Malaysia. PhD Dissertation. Kuala Lumpur: University of Malaysia, 1982.

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