Rice Science ›› 2021, Vol. 28 ›› Issue (5): 493-500.DOI: 10.1016/j.rsci.2021.07.009

• Research Paper • Previous Articles     Next Articles

Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production

Balija Vishalakshi1,6,#, Bangale Umakanth1,#, Ponnuvel Senguttuvel2, Makarand Barbadikar Kalyani1, Prasad Madamshetty Srinivas3, Rao Durbha Sanjeeva4, Yadla Hari5, Madhav Maganti Sheshu1()   

  1. 1Biotechnology Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, India
    2Hybrid Rice Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, India
    3Plant Pathology Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, India
    4Plant Physiology Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, India
    5Regional Agricultural Research Station, Professor Jayashankar Telangana State Agricultural University, Warangal 506006, India
    6Department of Biotechnology, Acharya Nagarjuna University, Guntur 522510, India
  • Received:2020-08-14 Accepted:2021-01-08 Online:2021-09-28 Published:2021-09-28
  • About author:

    #These authors contributed equally to this work

Abstract:

Varalu is an early maturing rice variety widely grown in the rainfed ecosystem preferred for its grain type and cooking quality. However, the yield of Varalu is substantially low since it is being affected by reproductive drought stress along with the blast disease. The genetic improvement of Varalu was done by introgressing a major yield QTL, qDTY12.1, along with two major blast resistance genes i.e. Pi54 and Pi1 through marker-assisted backcross breeding. Both traits were transferred till BC2 generation and intercrossing was followed to pyramid the two traits. Stringent foreground selection was carried out using linked markers as well as peak markers (RM28099, RM28130, RM511 and RM28163) for the targeted QTL (qDTY12.1), RM206 for Pi54 and RM224 for Pi1. Extensive background selection was done using genome-wide SSR markers. Six best lines (MSM-36, MSM-49, MSM-53, MSM-57, MSM-60 and MSM-63) having qDTY12.1 and two blast resistance genes in homozygous condition with recurrent parent genome of 95.0%?96.5% having minimal linkage drag of about 0.1 to 0.7 Mb were identified. These lines showed yield advantage under drought stress as well as irrigated conditions. MSM-36 showed better performance in the national coordinated trials conducted across India, which indicated that improved lines of Varalu expected to replace Varalu and may have an important role in sustaining rice production. The present study demonstrated the successful marker-assisted pyramiding strategy for introgression of genes/QTLs conferring biotic stress resistance and yield under abiotic stress in rice.

Key words: blast resistance, drought stress, marker-assisted gene pyramiding, rainfed ecosystem, upland rice