Biochemical Responses Associated with Resistance to Nilaparvata lugens (Stål) in Wild Rice Accessions

doi:10.1016/j.rsci.2020.09.002

Abstract

Kaur Sandhu Rajwinder, Singh Sarao Preetinder, Kumari Neelam. Biochemical Responses Associated with Resistance to Nilaparvata lugens (Stål) in Wild Rice Accessions[J]. Rice Science, 2020, 27(6): 449-453.

Figures/Tables 6

References 44

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Accession	Modified seed box score	Categorization on the basis of SES	Excreted honeydew area (mm²)	Weight of filter paper with honeydew (mg)	Watery lesion
IRGC99577 (O. punctata)	2.17 ± 0.30	Resistant	74.10 ± 1.10 a	9.50 ± 0.30 a	2.70 ± 0.10 e
IRGC105270 (O. australiensis)	3.43 ± 0.43	Resistant	101.50 ± 1.10 b	13.50 ± 0.50 b	2.50 ± 0.10 de
IRGC105275 (O. australiensis)	4.60 ± 0.40	Moderately resistant	123.70 ± 1.30 c	16.40 ± 0.20 c	2.10 ± 0.10 c
IRGC104646 (O. nivara)	2.85 ± 0.40	Resistant	113.90 ± 1.90 c	14.80 ± 0.40 bc	2.30 ± 0.10 cd
CR100204 (O. nivara)	5.03 ± 0.30	Moderately resistant	147.90 ± 6.30 d	20.00 ± 1.20 d	1.70 ± 0.10 b
Ptb33 (Resistant check)	2.30 ± 0.30	Resistant	78.60 ± 1.20 a	10.10 ± 0.30 a	2.60 ± 0.00 de
TN1 (Susceptible check)	9.00 ± 0.00	Susceptible	412.70 ± 4.30 e	52.40 ± 1.00 e	0.30 ± 0.10 a
LSD_0.05	1.12		0.84	2.21	0.31
SES, Standard evaluation system. Data are Mean ± SE (n = 30 for modified seed box score and 5 for honeydew area and watery lesion). Means within a column followed by the same lowercase letters are not significantly different at P ≤ 0.05 according to the Duncan’s multiple range test.

Accession	Modified seed box score	Categorization on the basis of SES	Excreted honeydew area (mm²)	Weight of filter paper with honeydew (mg)	Watery lesion
IRGC99577 (O. punctata)	2.17 ± 0.30	Resistant	74.10 ± 1.10 a	9.50 ± 0.30 a	2.70 ± 0.10 e
IRGC105270 (O. australiensis)	3.43 ± 0.43	Resistant	101.50 ± 1.10 b	13.50 ± 0.50 b	2.50 ± 0.10 de
IRGC105275 (O. australiensis)	4.60 ± 0.40	Moderately resistant	123.70 ± 1.30 c	16.40 ± 0.20 c	2.10 ± 0.10 c
IRGC104646 (O. nivara)	2.85 ± 0.40	Resistant	113.90 ± 1.90 c	14.80 ± 0.40 bc	2.30 ± 0.10 cd
CR100204 (O. nivara)	5.03 ± 0.30	Moderately resistant	147.90 ± 6.30 d	20.00 ± 1.20 d	1.70 ± 0.10 b
Ptb33 (Resistant check)	2.30 ± 0.30	Resistant	78.60 ± 1.20 a	10.10 ± 0.30 a	2.60 ± 0.00 de
TN1 (Susceptible check)	9.00 ± 0.00	Susceptible	412.70 ± 4.30 e	52.40 ± 1.00 e	0.30 ± 0.10 a
LSD_0.05	1.12		0.84	2.21	0.31
SES, Standard evaluation system. Data are Mean ± SE (n = 30 for modified seed box score and 5 for honeydew area and watery lesion). Means within a column followed by the same lowercase letters are not significantly different at P ≤ 0.05 according to the Duncan’s multiple range test.

Accession	PAL [μg/(h·mg)]				PPO [U/(min·mg)]
	Leaf blade		Leaf sheath		Leaf blade		Leaf sheath
	Constitutive level	Induced level	Constitutive level	Induced level	Constitutive level	Induced level	Constitutive level	Induced level
IRGC99577	2.23 ± 0.05 e	2.36 ± 0.05 c	1.91 ± 0.04 d	2.16 ± 0.07 d	0.19 ± 0.00 e	0.21 ± 0.01 e	0.16 ± 0.01 d	0.18 ± 0.01 d
IRGC105270	2.02 ± 0.05 d	2.17 ± 0.05 b	1.80 ± 0.03 c	1.94 ± 0.06 c	0.15 ± 0.02 cde	0.17 ± 0.01 cd	0.11 ± 0.00 c	0.13 ± 0.01 bc
IRGC105275	1.80 ± 0.03 bc	1.92 ± 0.02 a	1.51 ± 0.02 b	1.65 ± 0.04 b	0.13 ± 0.01 bc	0.15 ± 0.00 c	0.08 ± 0.00 b	0.12 ± 0.01 bc
IRGC104646	1.93 ± 0.06 cd	2.11 ± 0.06 b	1.71 ± 0.04 c	1.84 ± 0.03 c	0.15 ± 0.01 cd	0.17 ± 0.01 c	0.12 ± 0.01 c	0.13 ± 0.01 c
CR100204	1.75 ± 0.04 b	1.87 ± 0.04 a	1.45 ± 0.03 b	1.60 ± 0.04 b	0.11 ± 0.01 b	0.13 ± 0.01 b	0.08 ± 0.00 b	0.09 ± 0.01 ab
Ptb33	2.25 ± 0.04 e	2.40 ± 0.04 c	1.93 ± 0.03 d	2.20 ± 0.05 d	0.17 ± 0.01 de	0.19 ± 0.01 de	0.14 ± 0.01 d	0.17 ± 0.01 d
TN1	1.57 ± 0.02 a	1.76 ± 0.04 a	1.30 ± 0.02 a	1.39 ± 0.01 a	0.07 ± 0.01 a	0.09 ± 0.00 a	0.05 ± 0.00 a	0.06 ± 0.01 a
LSD_0.05
Accession (A)	0.05		0.04		0.09		0.09
Infestation (I)	0.10		0.08		0.02		0.18
A × I	Not significant		Not significant		Not significant		Not significant
Values are Mean ± SE (n = 3). Means within a column followed by the same lowercase letters are not significantly different at P ≤ 0.05 according to the Duncan’s multiple range test.

Accession	PAL [μg/(h·mg)]				PPO [U/(min·mg)]
	Leaf blade		Leaf sheath		Leaf blade		Leaf sheath
	Constitutive level	Induced level	Constitutive level	Induced level	Constitutive level	Induced level	Constitutive level	Induced level
IRGC99577	2.23 ± 0.05 e	2.36 ± 0.05 c	1.91 ± 0.04 d	2.16 ± 0.07 d	0.19 ± 0.00 e	0.21 ± 0.01 e	0.16 ± 0.01 d	0.18 ± 0.01 d
IRGC105270	2.02 ± 0.05 d	2.17 ± 0.05 b	1.80 ± 0.03 c	1.94 ± 0.06 c	0.15 ± 0.02 cde	0.17 ± 0.01 cd	0.11 ± 0.00 c	0.13 ± 0.01 bc
IRGC105275	1.80 ± 0.03 bc	1.92 ± 0.02 a	1.51 ± 0.02 b	1.65 ± 0.04 b	0.13 ± 0.01 bc	0.15 ± 0.00 c	0.08 ± 0.00 b	0.12 ± 0.01 bc
IRGC104646	1.93 ± 0.06 cd	2.11 ± 0.06 b	1.71 ± 0.04 c	1.84 ± 0.03 c	0.15 ± 0.01 cd	0.17 ± 0.01 c	0.12 ± 0.01 c	0.13 ± 0.01 c
CR100204	1.75 ± 0.04 b	1.87 ± 0.04 a	1.45 ± 0.03 b	1.60 ± 0.04 b	0.11 ± 0.01 b	0.13 ± 0.01 b	0.08 ± 0.00 b	0.09 ± 0.01 ab
Ptb33	2.25 ± 0.04 e	2.40 ± 0.04 c	1.93 ± 0.03 d	2.20 ± 0.05 d	0.17 ± 0.01 de	0.19 ± 0.01 de	0.14 ± 0.01 d	0.17 ± 0.01 d
TN1	1.57 ± 0.02 a	1.76 ± 0.04 a	1.30 ± 0.02 a	1.39 ± 0.01 a	0.07 ± 0.01 a	0.09 ± 0.00 a	0.05 ± 0.00 a	0.06 ± 0.01 a
LSD_0.05
Accession (A)	0.05		0.04		0.09		0.09
Infestation (I)	0.10		0.08		0.02		0.18
A × I	Not significant		Not significant		Not significant		Not significant
Values are Mean ± SE (n = 3). Means within a column followed by the same lowercase letters are not significantly different at P ≤ 0.05 according to the Duncan’s multiple range test.

Accession	Total phenol (mg/g)				Flavonol (mg/g)				Total free amino acid (mg/g)
	Leaf blade		Leaf sheath		Leaf blade		Leaf sheath		Leaf blade		Leaf sheath
	Constitutive level	Induced level	Constitutive level	Induced level	Constitutive level	Induced level	Constitutive level	Induced level	Constitutive level	Induced level	Constitutive level	Induced level
IRGC99577	6.18 ± 0.06 d	6.36 ± 0.05 d	2.63 ± 0.08 d	2.87 ± 0.03 c	2.49 ± 0.07 e	2.97 ± 0.06 e	0.73 ± 0.07 ab	1.27 ± 0.04 b	1.15 ± 0.12 a	1.30 ± 0.12 a	1.11 ± 0.10 a	1.26 ± 0.13 a
IRGC105270	5.58 ± 0.09 c	5.94 ± 0.05 c	2.16 ± 0.06 c	2.72 ± 0.04 c	1.85 ± 0.13 cd	2.61 ± 0.07 d	0.67 ± 0.08 ab	1.08 ± 0.16 ab	1.44 ± 0.12 abc	1.69 ± 0.11 abc	1.23 ± 0.11 a	1.44 ± 0.15 ab
IRGC105275	5.06 ± 0.06 b	5.30 ± 0.04 b	1.87 ± 0.04 b	2.25 ± 0.07 b	1.68 ± 0.07 bc	1.99 ± 0.06 b	0.62 ± 0.07 ab	0.95 ± 0.09 ab	1.53 ± 0.09 bc	1.80 ± 0.11 bcd	1.25 ± 0.11 a	1.47 ± 0.16 ab
IRGC104646	5.97 ± 0.04 d	6.21 ± 0.06 d	2.18 ± 0.06 c	2.78 ± 0.03 c	2.02 ± 0.04 d	2.34 ± 0.05 c	0.64 ± 0.07 ab	1.02 ± 0.13 ab	1.44 ± 0.11 abc	1.67 ± 0.13 abc	1.22 ± 0.10 a	1.42 ± 0.13 ab
CR100204	4.61 ± 0.07 a	5.16 ± 0.07 b	1.79 ± 0.07 b	2.09 ± 0.07 b	1.49 ± 0.14 b	1.87 ± 0.13 b	0.57 ± 0.05 ab	0.82 ± 0.08 a	1.52 ± 0.07 bc	1.85 ± 0.13 cd	1.31 ± 0.08 a	1.56 ± 0.12 ab
Ptb33	7.62 ± 0.11 e	7.79 ± 0.13 e	2.72 ± 0.07 d	3.77 ± 0.05 d	2.75 ± 0.06 e	3.07 ± 0.06 e	0.78 ± 0.07 b	1.30 ± 0.06 b	1.24 ± 0.07 ab	1.40 ± 0.08 ab	1.16 ± 0.06 a	1.32 ± 0.10 ab
TN1	4.35 ± 0.10 a	4.90 ± 0.03 a	1.42 ± 0.09 a	1.69 ± 0.06 a	1.15 ± 0.08 a	1.52 ± 0.07 a	0.52 ± 0.05 a	0.83 ± 0.11 a	1.70 ± 0.09 c	2.14 ± 0.13 d	1.42 ± 0.11 a	1.76 ± 0.13 b
LSD (P = 0.05)
Accession	0.08		0.07		0.10		0.10		0.12		0.13
Infestation	0.16		0.13		0.18		0.19		0.22		NS
Accession × Infestation	NS		0.19		NS		NS		NS		NS

Biochemical Responses Associated with Resistance to Nilaparvata lugens (Stål) in Wild Rice Accessions

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Regression equation	Coefficient of determination (R²)
Y₁ = -51.05 - 17.39X₁ + 208.48X₂ - 24.87X₃ + 105.65X₄ + 37.08X₅	0.99
Y₂ = -2156.32 + 193.31X₃ - 267.66X₄+ 1667.12X₅	0.81
Y₃ = 19.06 - 18.75X₂ + 1.14X₃ - 3.29X₄ - 12.32X₅	0.92
Y₁ = Damage score, Y₂ = Honeydew excretion, Y₃ = Ovicidal response, X₁ = Phenylalanine ammonia lyase, X₂ = Polyphenol oxidase, X₃ = Total phenols, X₄ = Flavonols, X₅ = Free amino acids.