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    Forty Years’ Development of Hybrid Rice: China’s Experience
    CHENG Shi-hua , CAO Li-yong , YANG Shi-hua , ZHAI Hu-qu
    2004, 11(5-6): 225-230 . 
    Abstract ( )   PDF (48KB) ( )  
    China is the first country where heterosis of hybrid rice was successfully exploited commercially on a large scale in the world. Hybrid rice has been developed for 40 years since Prof. Yuan initiated it in China. It had been planted about 330 million hectares with an increase of nearly 400 million tons of rice during 1976–2002. China’s experience on hybrid rice could be attributed to utilization of various cytoplasmic male sterility (CMS) resources, high outcrossing rate CMS lines and stable environmentally induced genetic male sterile (EGMS) lines, improvement of diseases resistance and grain quality, and combination of ideal plant type with heterosis in hybrid rice breeding program. Innovative breeding techniques, e.g. improvement in root system, molecular marker-assisted selection and wild hybridization should be considered in further development of hybrid rice in China.
    Research Paper
    Improvement of Resistance to Bacterial Blight by Marker-Assisted Selection in a Wide Compatibility Restorer Line of Hybrid Rice
    LUO Yan-chang, WANG Shou-hai, LI Cheng-quan, WU Shuang, WANG De-zheng, DU Shi-yun
    2004, 11(5-6): 231-237 . 
    Abstract ( )   PDF (46KB) ( )  
    4183 is a promising wide compatibility restorer line with good grain quality. Its hybrid rice Shuangyou 4183 (Shuangjiu A/ 4183) was registered in Anhui Province in 2003. However, the line and its hybrid rice are susceptible to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo). R4183 carrying Xa21 was developed to improve bacterial blight resistance of 4183 through introducing the broad-spectrum resistance gene Xa21 by marker-aided selection. R4183 had similar level of bacterial blight resistance to IRBB21, the resistant donor, while maintained the wide compatibility, restoring ability and other good economic traits of the recurrent parent 4183. Critical issues on improvement of bacterial blight resistance of hybrid rice and breeding strategies were also discussed.
    Quantitative Trait Loci and Epistatic Analysis of Seed Anoxia Germinability in Rice (Oryza sativa)
    JIANG Ling, HOU Ming-yu, WANG Chun-ming, WAN Jian-min
    2004, 11(5-6): 238-244 . 
    Abstract ( )   PDF (79KB) ( )  
    Anoxia germinability (AG) of 35 rice varieties was evaluated under different temperature and water submergence conditions. The shoot (including coleoptile) length of seedlings germinating under 30℃, 0.2 m water submergence for 5 days could be used as an optimal criterion for the AG evaluation of all the varieties. Differences were observed among the AGs of 359 varieties from different regions and subspecies with the optimized method. Moreover, 81 recombinant inbred lines derived from a cross of Kinmaze (japonica)/DV85 (indica) were used to detect quantitative trait loci (QTLs) conferring AG. A total of five QTLs for AG in the recombinant inbred population were detected on chromosomes 1, 2, 5 and 7, respectively. Phenotypic variations explained by each QTL ranged from 10.5% to 19.6%. Based on the directions of the additive effects, the alleles at three loci qAG-1, qAG-2 and qAG-7 from Kinmaze increased AG, while alleles at loci qAG-5a and qAG-5b from DV85 increased AG. Meanwhile, three pairs of epistatic loci were found to be located on chromosomes 2, 3, 5 and 11 with significant effects ranging from 16.7% to 48.8%, and the highest one 48.78%, was detected between C563–X182 on chromosome 3 and R830–X208 on chromosome 5.
    QTLs Analysis of Cold Tolerance During Early Growth Period for Rice
    HAN Long-zhi , QIAO Yong-li , CAO Gui-lan , ZHANG Yuan-yuan , AN Yong-ping , YE Jong-doo , KOH Hee-jong
    2004, 11(5-6): 245-250 . 
    Abstract ( )   PDF (62KB) ( )  
    The quantitative trait loci (QTLs) for cold tolerance during early growth period were identified using a F2:3 population
    (including 200 individuals and lines derived from a cross between indica rice and japonica rice ‘Milyang 23/Jileng 1’) with
    microsatellite markers. The cold tolerance at the seedling and tillering stages, and the growth ability of seedling under low
    temperature conditions were evaluated. All of the traits associated with cold tolerance at early growth stages appeared a continuous
    distribution near to normal in F3 lines, these were inherited as quantitative traits controlled by polygenes. Three QTLs on
    chromosomes 1, 5 and 9, which associated with cold tolerance at the seedling stage were detected. Among them, qCTS1
    accounted for 15.5% of observed phenotypic variation; Five QTLs on chromosomes 2, 3, 7, 9 and 11, associated with cold tolerance
    at the tillering stage were found, which explained lower percentage of observed phenotypic variation; Four QTLs on chromosomes 1,
    2, 11 and 12, which associated with the growth ability of seedling under low temperature conditions were found, among them,
    qGAS2 and qGAS12 explained 26.6 and 42.9% of observed phenotypic variation, respectively, which were major genes.
    Tagging Blast Resistance Gene Pi 1 in Rice (Oryza sativa) Using Candidate Resistance Genes
    WU Jian-li, Menchu BERNADO, ZHUANG Jie-yun, ZHENG Kang-le, Hei LEUNG
    2004, 11(5-6): 251-254 . 
    Abstract ( )   PDF (70KB) ( )  
    An F3 population derived from C101LAC/CO39 containing 90 lines was analyzed for blast resistance with 48 candidate genes developed from resistance gene analogs (RGA) and suppression subtractive library. Genetic analysis confirmed that blast resistance of the population was controlled by a single gene Pi 1. One of the candidate genes, R10 was identified as associated with the blast resistance gene on the long arm of chromosome 11 and mapped using a DH population derived from Azucena/IR64. A pair of PCR based primers was designed based on the sequence of R10 for marker-aided selection of the blast resistance gene. The recombination frequency between Pi 1 and the marker was estimated as 1.28%. It suggested that strategy of employing candidate genes is useful for gene identification and mapping. A new RFLP marker and the corresponding PCR marker for tagging of Pi 1 were provided.
    Genetic Analysis of Heterosis for Number of Spikelets per Panicle and Panicle Length of F1 Hybrids in japonica Rice Hybrids
    HONG De-Lin, LENG Yan
    2004, 11(5-6): 255-260 . 
    Abstract ( )   PDF (49KB) ( )  
    Genetic analysis of heterosis for number of spikelets per panicle and panicle length of F1 hybrid was conducted by using japonica rice varieties Bing 8979, C Bao, their F1, F2 and triple test cross (TTC) progenies. The two traits, panicle length and number of spikelets per panicle were controlled by polygenes, which were dispersed in the two parents. The dispersion of these polygenes was the genetic basis for the heterosis. Genetic variation in panicle length was mainly due to additive and dominant effects, and the dominant component played a determinative role. For number of spikelets per panicle, the effect of non-allelic genes was highly significant (1% probability level), and there existed epistasis including effects of additive×additive, additive×dominant, and dominant×dominant.
    Cold Tolerance of Core Collection at Booting Stage Associated with Eco-geographic Distribution in Yunnan Rice Landrace (Oryza sativa), China
    LI Shen-chong , ZENG Ya-wen , SHEN Shi-quan , PU Xiao-ying
    2004, 11(5-6): 261-268 . 
    Abstract ( )   PDF (48KB) ( )  
    Abstract: Four hundred and seventy-seven accessions of the second core collection of rice landrace from five rice cropping regions (16 prefectures) of Yunnan Province, China, were evaluated for cold tolerance at booting stage. The results showed as follows: 1) there was a significant positive correlation (0.588**) between anther length and seed setting rate under natural low temperature conditions but was not significant in greenhouse (0.080). 2) there were significant differences in cold tolerance among core collections from different cropping regions under natural low temperature conditions while a negligible cold damage in greenhouse (with a seed setting rate approaching or exceeding 70%) for cold tolerance evaluation. Cold tolerance of core collection from Northwest Yunnan cold highland japonica region was the strongest, and that from south marginal paddy-upland rice region was the most sensitive, suggesting that the breeding goals for cold tolerance should be different in various rice cropping regions. 3) there were remarkable differences in cold tolerance of core collections from different prefectures of Yunnan Province under natural low temperature conditions. Based on the reduction of seed setting rate and characteristics of natural climate, the 16 prefectures could be divided into three categories, i.e. serious cold damage , cold damage and slight cold damage regions. 4) Difference of cold tolerance between different rice cropping regions and prefectures revealed further that temperature change caused by elevation and latitude was not only a dominant factor for differentiation of japonica and indica but also the basic reason that the genetic diversity and six ecological group of indica and japonica were being developed and, even the critical factor leading to the formation of the cold tolerance gene as well. The cold tolerance at booting stage could be believed to be resulted from the long term co-evolution between Yunnan rice landrce and cold stress in rice cropping regions.
    Effect of System of Rice Intensification on Grain Plumpness in Association with Source-Sink Ratio in Mid-Season Hybrid Rice
    XU Fu-xian, XIONG Hong, ZHU Yong-chuan, WANG Gui-xiong
    2004, 11(5-6): 269-273 . 
    Abstract ( )   PDF (33KB) ( )  
    Grain plumpness under two cultivation methods, the system of rice intensification(SRI) and traditional cultivation, was examined using 18 mid-season hybrid rice combinations. There existed a highly significant negative correlation between the differences of grain plumpness under the two cultivation methods and number of spikelets per panicle. The small- or middle-panicle type hybrid rice though they showed a significant decrease in leaf-grain ratio under SRI maintained normal grain filling due to plenty of source supply, while the large-panicle hybrid rice was on the contrary. It suggested that the number of spikelets per panicle below 173 under traditional cultivation, was an index for selecting variety in application of SRI in the southern region of Sichuan Province, China.
    Change Law of Hyperspectral Data in Related with Chlorophyll and Carotenoid in Rice at Different Developmental Stages
    TANG Yan-lin, HUANG Jing-feng, WANG Ren-chao
    2004, 11(5-6): 274-282 . 
    Abstract ( )   PDF (258KB) ( )  
    The hyperspectral reflectances of the canopy, the first and the third unfolding leaves from the top and the panicles of two rice varieties (Xiushui 110 and Xieyou 9308) were measured by a ASD FieldSpec Pro FRTM in field and indoor environments under three nitrogen levels at different developmental stages. The concentrations of chlorophyll and carotenoid in leaves and panicle corresponding to the spectra were determined by biochemical method. The spectral differences were significant for rice under different nitrogen levels, and the concentrations of chlorophyll and carotenoid in leaves increased along with increasing applied nitrogen. There were more pronounced differences for the pigment concentrations in rice leaves with different nitrogen levels. The spectral reflectance of canopy was gradually getting smaller in the visible region and bigger in the near infrared region as the growth stages followed before heading, but with reverse change after heading. There existed ‘blue shift’ phenomena for the position of red edge in the spectra of canopy, leaves and panicle after heading. The concentrations of chlorophyll and carotenoid in leaves presented S-shape change. The concentrations of chlorophyll and carotenoid in canopies, leaves and panicles were highly significantly correlated to the hyperspectral vegetation indices (Vis) R990/R553, R1200/R553, R750/R553, R553/R670, R800/R553, PSSRa, PSNDa and the red edge positionλred, indicating that these VIs could be used to estimate the concentrations of chlorophyll and carotenoid in canopies, leaves and panicles of rice.
    Characteristics of Gas Exchange and Chlorophyll Fluorescence in Different Position Leaves at Booting Stage in Rice Plants
    JIN Song-heng, WANG Pin-mei, ZHAO Kai, YANG Yi-qing, YAO Sheng, JIANG De-an
    2004, 11(5-6): 283-289 . 
    Abstract ( )   PDF (85KB) ( )  
    To investigate the senescence of rice leaves at different positions at booting stage, gas exchange and chlorophyll fluorescence properties in leaves at different positions were examined by using six rice materials. The net rates of photosynthesis (Pn), stomatal conductance (gs), transpiration rate (E) and water use efficiency (WUE) decreased significantly with lowering of leaf positions, while intercellular CO2 concentration (Ci) had a little change, indicating that the decrease of photosynthetic rate was not resulted from the decrease of gs. The decrease of SPAD reading which had a close correlation with chlorophyll content was one of apparent reasons resulting in the decease of Pn. Further evidence by chlorophyll fluorescence showed that the photochemical efficiency of PSⅡ and its electron transport rate (ETR) decreased substantially with lowering of the leaf positions but the variable-to-maximum fluorescence ratio (Fv/Fm) and efficiency of energy conversion of open PSⅡ (Fv’/Fm’) remained rather stable, suggesting that primary limitation of Pn was suppression of electron transport of PSⅠ, and probably associated with carbon assimilation.
    Effects of Bicarbonate and High pH Conditions on Zinc and Other Nutrients Absorption in Rice
    MENG Fan-hua, WEI You-zhang, YANG Xiao-e, LIN Jian-jun, LIU Jian-xiang
    2004, 11(5-6): 290-296 . 
    Abstract ( )   PDF (57KB) ( )  
    Zinc deficiency was widely observed in calcareous soil where bicarbonate and high pH were always related with low zinc availability. In a hydroponic experiment, one zinc-efficient rice (IR36) and one zinc-inefficient rice (IR26) genotypes were employed to investigate the effects of bicarbonate and high pH conditions on absorption, transport of zinc and other nutrients (P, K, Ca, Mg, Fe, Cu, Mn) in rice. As compared with the control, high pH inhibited absorption, translocation and accumulation of zinc and other nutrients in both rice genotypes. Bicarbonate had minor effect on zinc-efficient rice genotype (IR36) whereas it could decrease zinc and other nutrient absorption in zinc-inefficient rice genotype (IR26). These results implied that increasing rice tolerance to bicarbonate is one of the most important strategies to improve rice adaptation for zinc-deficit calcareous soil.
    Monitoring of Insecticide Resistance and Genetic Analysis of Triazophos Resistance in Chilo suppressalis (Lepidoptera: Pyralidae)
    CAO Ming-zhang, SHEN Jin-liang, ZHANG Jin-zhen, LU Mei, LIU Xiao-yu, ZHOU Wei-jun
    2004, 11(5-6): 297-304 . 
    Abstract ( )   PDF (76KB) ( )  
    During 2001 and 2002, insecticide resistance in the fourth instar larvae of striped stem borer (Chilo suppressalis), which were collected from Zhejiang, Jiangsu, Anhui and Jiangxi provinces in China, was monitored using topical application method. Low level of resistance to fipronil (6.5-fold) was detected for the first time in RA (Rui’an) population from southeast Zhejiang, but the other six populations tested remained susceptible to this recently introduced insecticide. No resistance to abamectin had been found after examining six populations from Zhejiang, Jiangsu and Anhui provinces. Resistance to triazophos was monitored in ten populations from the four Provinces, and very high level resistance(163.1-fold) was found in RA population, moderate (18.2-fold) in WZ (Wenzhou, Zhejiang Province) population, and low (6.7- to 9.7-fold) in populations of CS (Changshu), XS (Xishan) and JT (Jintan) from south Jiangsu, whereas the other five populations were susceptible. All the nine populations monitored were resistant to monosultap with varying degree, i.e. high level (113.7- and 57.6-fold, respectively) of resistance in RA and YF (Yifeng, Jiangxi Province) populations, moderate (11.0- to 29.7-fold) in WZ, CS, JT and TH (Taihu, Anhui Province) populations, low (6.7- and 7.5-fold, respectively) in XY (Xinyang, Jiangsu Province) and XS populations, and the lowest (3.7-fold) in GY (Guanyun, Jiangsu Province) population. Inheritance of resistance in triazophos selected strain Rts was studied through reciprocal cross and backcross experiments. The preliminary results indicated that inheritance of triazophos resistance in Rts strain was incompletely dominant, with degrees of dominance being 0.46 and 0.68 for reciprocal crosses, and that the resistance was controlled by a major gene, though minor modifying gene(s) might be involved.
    Effect of Rice Volatiles on the Orientation Behavior of the Striped Stem Borer, Chilo suppressalis Larvae
    CHEN Hua-cai , LOU Yong-gen , CHENG Jia-an , SHEN Qun-chao
    2004, 11(5-6): 305-308 . 
    Abstract ( )   PDF (38KB) ( )  
    The orientation behavior of the 1st and the 3rd instar larvae of the striped stem borer (SSB), Chilo suppessalis, to the volatiles emitted from rice plants was studied with a Y-tube olfactometer. The treatments included healthy plants vs clean air (HP vs CA), striped stem borer damaged plants vs clean air (SSB-DP vs CA), rice leaf folder damaged plants vs clean air (RLF-DP vs CA), HP vs SSB-DP, HP vs RLF-DP and SSB-DP vs RLF-DP. Volatiles from all rice plants had significant attraction for both of the 1st and the 3rd instar larvae. The orientation selection of larvae to the volatiles from rice plants treated differentially (HP, SSB-DP, and RLF-DP) did not impose any significant influence.
    Changes in Methamidophos Resistance and Fitness of Hybrids in Different Strains of Nilaparvata lugens
    LIU Ze-wen, HAN Zhao-jun, ZHANG Ling-chun
    2004, 11(5-6): 309-312 . 
    Abstract ( )   PDF (45KB) ( )  
    A resistant strain selected successively in the laboratory for 17 generations had 198.63-fold resistance to methamidophos. The resistant levels and fitness of progenies from the resistant strain and susceptible strain or field population were closer to those of the resistant strain than those of the susceptible strain or field population. The changes in the resistant levels of the hybrid were propitious to the resistance development, however, the changes of the fitness went to the contrary. The effects of the migration on the development of methamidophos resistance in Nilaparvata lugens were discussed in the aspects of the migration of Nilaparvata lugens, the resistant levels of progenies and the changes of the fitness.
    Anti-Feedant Activity of the Extracts from Six Species of Wild Rice Against Spodoptera litura
    WAN Shu-qing, FENG Guo-zhong, PAN Da-jian, LUO Qing, DENG Jian-chao
    2004, 11(5-6): 313-316 . 
    Abstract ( )   PDF (35KB) ( )  
    Eight wild rice entries related to six species, including E6-1 and E6-3/6-4 (different ecotypes of Oryza grandiglumis); E7-4 (O. granulata), E9-10 and E9-20(different ecotypes of O. latifolia), E13-13 (O. minuta) , E15-13 (O. officinalis) and E16-3 (O. punctata), were used in experiment for the investigation of their anti-feedant effect on Spodoptera litura. The rice leaf extracts were prepared with methanol by Sohxlet method. The extract rates of the six species of wild rice were between 8.50% and 24.86%. Selective anti-feedant activity and non-selective anti-feedant activity of the extracts from the six species of wild rice against the 3rd instar larvae of S. litura showed that the extract of the wild rice E6-1 of O. grandiglumis had stronger anti-feedant activity than the others. The concentration for 50% anti-feedant activity (AFC50) of the wild rice E6-1 extract against the 2nd instar larvae of S. litura was 120 mg/mL in 24 h and 50 mg/mL in 48 h after treatment.
    Detection of Sensitivity and Resistance Variation of Magnaporthe grisea to Kitazin P, Carbendazim and Tricyclazole
    ZHANG Chuan-qing, ZHOU Ming-guo, SHAO Zhen-run, LIANG Gui-mei
    2004, 11(5-6): 317-323 . 
    Abstract ( )   PDF (63KB) ( )  
    One hundred and twenty-nine isolates of Magnaporthe grisea from Guangdong, Guangxi, Anhui and Jiangsu provinces of China were tested for resistance frequency to kitazin P and carbendazim, respectively by the distinctive concentration method. The resistance frequency of the isolates to kitazin P which had not been used in practice for about ten years was as high as 79.1%, and only one carbendazim-resistant isolate was detected in Gaoyao, Guangdong Province (with a frequency of 0.78%). Meanwhile, the minimum inhibition concentration (MIC) of hyphal melanization was adopted to detect the sensitivity of M. grisea to tricyclazole. There existed several different degrees of sensitivity to tricyclazole in the melanin biosynthesis of M. grisea, but no relationship was found between these MIC values completely inhibiting melanization in hyphae and the EC50 values of tricyclazole against rice blast tested in vivo. After the isolates were induced by chemical taming or UV irradiation in laboratory, kitazin P-resistant and carbendazim-resistant mutants were recovered by both the methods, but none of tricyclazole-resistant mutant was obtained.
    Distribution of Magnaporthe grisea Population and Virulence of Predominant Race in Jiangsu Province, China
    LIU Yong-feng, CHEN Zhi-yi, HU Ming, LI Lian, LIU You-zhou
    2004, 11(5-6): 324-330 . 
    Abstract ( )   PDF (50KB) ( )  
    Three hundred and forty-two mono­-conidial isolates were obtained from rice blast specimens collected from five typical areas in Jiangsu Province during 2000-2002. The isolates could be classified into seven groups, thirty races when assessed with seven Chinese differential rice cultivars. The race ZG1 was predominant one of Magnaporthe grisea with a frequency of 65.00% in 2000, 56.90% in 2001 and 60.38% in 2002; the races ZB and ZC groups were also important in Jiangsu Province. By inoculating 130 ZG1 race isolates on thirteen Japanese cultivars with known resistance genes, forty-two pathotypes were found. Among them, 30.77% of ZG1 race isolates had virulence to rice cultivars Shin 2(Pita-ks, Pi-sh), K1(Pi-ta), Pi 4(Pi-ta2, Pi-sh), being the predominant pathotype, indicating the resistances of cultivars Shin 2 (Pita-ks, Pi-sh), K1(Pi-ta), Pi 4(Pi-ta2, Pi-sh) had lost the resistance in Jiangsu Province. Rice cultivar K3(Pi-kh) was highly resistant to Magnaporthe grisea with 100% resistance frequency, and resistant to the two single isolates, 2003-184(ZC5) and 2003-14-1(ZG1), however, it was infected by a mixture of the two isolates. It was suggested that the change in virulence stemmed from the interaction of different pathotype isolates was one of the reasons that made variety lose its resistance.
    A Study on Tolerance to Pretilachlor in Eight Species of Echinochloa
    WANG Qing-ya, QIAO Li-ya, WEI Jie-gang, DONG Li-yao , LI Yang-han
    2004, 11(5-6): 331-335 . 
    Abstract ( )   PDF (68KB) ( )  
    Height of seedlings, length of leaf and coleoptile cells, and α-amylase activities of eight species or varieties of barnyardgrass (Echinochloa) were measured after seeds being treated with pretilachlor. There existed obvious differences in the tested traits among the materials. The inhibition rate of pretilachlor to Echinochloa crusgalli (L.) Beavu. var. mitis (Pursh.) Peterm was the lowest, and that to E. colonum (L.) Link was the highest among the eight species or varieties of barnyardgrass. It suggested that the different species or varieties of barnyardgrass possess variable tolerance to pretilachlor.
    Effect of Indica Rice Diets Supplemented with Exogenous Enzyme on Growth and Digestion of Pigs Fed Paddy-Based Diets
    WANG Min-qi, XU Zi-rong, SUN Jian-yi
    2004, 11(5-6): 336-341 . 
    Abstract ( )   PDF (50KB) ( )  
    Sixty growing pigs (Landrace × Jiaxing black) were used to study the effects of exogenous enzymes (including β-glucanase, xylanase and cellulase) supplemented to paddy-based diets on pig growth performance and digestion. With the supplementation of enzymes, average daily gain of pig was increased by 8.78%(P<0.05), feed gain radio was decreased by 9.42%(P<0.05), feed apparent digestibilities of crude protein, crude fat and crude fiber were increased by 17.32% (P<0.01), 16.04% (P<0.05) and 108.57%(P<0.01), the viscosity of jejunum, and ileum contents were reduced by 7.08%(P<0.01) and 6.78%(P<0.01), numbers of E. coli in feaces and diarrhoea morbidity were decreased by 81.29%(P<0.01) and 37.00%(P<0.05), and the activities of protelytic enzyme and a-amylase in duodenal contents were enhanced by 99.07% (P<0.05) and 18.38%(P<0.05), respectively.
    Experimental Technique
    A One-Step PCR Method for Detecting the First Base of Splice Donor of Wx Intron 1 in Rice
    MAO Xing-xue, LIU Yan-zhuo, XIAO Xin, CHEN Jian-wei, LUO Wen-yong, LI Xiao-fang
    2004, 11(5-6): 342-344 . 
    Abstract ( )   PDF (40KB) ( )  

    A new method of one-step PCR was devised for detecting the first nucleotide in the splice donor site of Wx intron 1. compared to the regular PCR-AccⅠmethod, the method can produce the same result for detecting +1 nucleotide of Wx intron 1. The reliability of the new method was confirmed with 30 rice varieties. The new technique is more convenient and cheaper than the regular PCR-AccⅠmethod, and could be widely deploded in rice molecular marker assistant selection.

    Short Communication
    Assessment of Various Factors Involved in the Tissue Culture System of Rice
    Shaukat ALI , XUE Qing-zhong , ZHANG Xian-yin
    2004, 11(5-6): 345-349 . 
    Abstract ( )   PDF (40KB) ( )  
    To optimize conditions for an efficient embryogenic callus induction and plant regeneration from mature seeds of japonica rice cultivar Xiushui 11 and indica cultivar XC95, different factors such as plant growth regulators, casein hydrolysate, carbohydrate source and concentration, and various gelation conditions including gel type for callus induction and phyta gel concentration for shoot regeneration were studied in detail to determine their role in the system. Overall, induction of embryogenic callus in case of Xiushui 11 was the most efficient (67.2%) in the media supplemented with 2 mg/L 2,4-D, 3% sucrose and 0.8% agar while in case of XC95, 2 mg/L of 2,4-D combined with 0.5 mg/L of BAP and NAA generated the best result (61.2%). Inclusion of 0.3% agarose (pure form) or phyta gel further enhanced the callus induction efficiency (80%) in Xiusui 11. Higher concentration (300–500 mg/L) of casein hydrolysate was found to significantly inhibit the browning of calli in XC95. Role of cytokinin and phyta gel concentration was critical for shoot regeneration in both classes of rice. Increased strength of phyta gel was observed to enhance the shoot regeneration frequency. The optimal concentration of 2 mg/L and 3 mg/L for KT was necessary for higher differentiation frequency supplemented with 0.5% and 0.6% phyta gel in case of japonica and indica rice, respectively.