Genetic Improvement of Japonica Rice Variety Wuyujing 3 for Stripe Disease Resistance and Eating Quality by Pyramiding Stv-bi and Wx-mq

doi:10.1016/j.rsci.2016.02.002

Abstract

Abstract:

Japonica rice variety Kanto 194 as the donor of resistance gene Stv-bⁱ and low-amylose content gene Wx-mq was used to improve the resistance and eating quality of Wuyujing 3 by the breeding strategy of backcross. In continuous backcross and selfcross generations, the related molecular markers with Stv-bⁱ and Wx-mq genes were utilized for genotypic detection by associated with resistance identification of rice stripe disease and agronomic traits selection. Finally, 10 improved lines with homozygous genotype Stv-bⁱStv-bⁱWx-mqWx-mq were obtained from BC₃F₄ generation. The results of comparative analysis indicated that most characters of these lines were consistent with those of recipient parent Wuyujing 3, except for the improved resistance, appearance and eating quality. By evaluation of the comprehensive performance of them, two excellent lines K01 and K04 were selected for further experiments.

Key words: rice, stripe disease resistance, eating quality, marker-assisted pyramiding selection, genetic improvement

Tao Chen, Hao Wu, Ya-dong Zhang, Zhen Zhu, Qi-yong Zhao, Li-hui Zhou, Shu Yao, Ling Zhao, Xin Yu, Chun-fang Zhao, Cai-lin Wang. Genetic Improvement of Japonica Rice Variety Wuyujing 3 for Stripe Disease Resistance and Eating Quality by Pyramiding Stv-bⁱ and Wx-mq[J]. Rice Science, 2016, 23(2): 69-77.

Figures/Tables 8

References 22

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Line/Variety	Incident rate of rice stripe disease (%)		Resistance level
Line/Variety	Nanjing, Jiangsu Province	Yandu, Jiangsu Province	Resistance level
K01	0.00 ± 0.00	0.20 ± 0.20	Highly resistant
K02	1.41 ± 0.21	1.55 ± 0.30	Highly resistant
K03	1.15 ± 0.31	1.48 ± 0.32	Highly resistant
K04	0.20 ± 0.00	0.41 ± 0.20	Highly resistant
K05	0.26 ± 0.23	0.94 ± 0.23	Highly resistant
K06	0.26 ± 0.11	0.87 ± 0.23	Highly resistant
K07	2.73 ± 0.50	3.74 ± 0.51	Highly resistant
K08	0.07 ± 0.12	0.40 ± 0.20	Highly resistant
K09	0.88 ± 0.31	1.48 ± 0.31	Highly resistant
K10	0.79 ± 0.39	1.28 ± 0.12	Highly resistant
Kanto 194	0.28 ± 0.11	0.66 ± 0.18	Highly resistant
Wuyujing 3	64.82 ± 0.80	68.97 ± 0.41	Highly susceptible

Line/Variety	Incident rate of rice stripe disease (%)		Resistance level
Line/Variety	Nanjing, Jiangsu Province	Yandu, Jiangsu Province	Resistance level
K01	0.00 ± 0.00	0.20 ± 0.20	Highly resistant
K02	1.41 ± 0.21	1.55 ± 0.30	Highly resistant
K03	1.15 ± 0.31	1.48 ± 0.32	Highly resistant
K04	0.20 ± 0.00	0.41 ± 0.20	Highly resistant
K05	0.26 ± 0.23	0.94 ± 0.23	Highly resistant
K06	0.26 ± 0.11	0.87 ± 0.23	Highly resistant
K07	2.73 ± 0.50	3.74 ± 0.51	Highly resistant
K08	0.07 ± 0.12	0.40 ± 0.20	Highly resistant
K09	0.88 ± 0.31	1.48 ± 0.31	Highly resistant
K10	0.79 ± 0.39	1.28 ± 0.12	Highly resistant
Kanto 194	0.28 ± 0.11	0.66 ± 0.18	Highly resistant
Wuyujing 3	64.82 ± 0.80	68.97 ± 0.41	Highly susceptible

Line/Variety	PH (cm)	PL (cm)	PP	FGPL	SPL	FGPA	SPA	SSR (%)	TGW (g)	GWP (g)
K01	97.9	15.7	14.3	1 397.9	1 452.2	98.6	102.6	96.3	25	33.6
K02	99.4	15	14.6	1 421.1	1 475.3	97.2	101	96.2	24.7	32.7
K03	99.1	15.4	14.3	1 289.6	1 354.7	90.8	95.5	95.2	24.7	31.2
K04	99.8	15.9	14.9	1 336.7	1 383.6	89.3	92.6	96.5	24.9	32.3
K05	99.7	15.5	13.4	1 249.0	1 291.6	93.6	96.8	96.7	25.9	32.1
K06	101.5	15.3	13.9	1 324.7	1 372.3	95.3	98.8	96.4	25.5	33.7
K07	100.2	15.9	13.4	1 288.7	1 329.5	96.3	99.4	96.9	25.1	32.2
K08	100.2	15.1	14	1 322.9	1 390.8	94.7	99.6	95.1	25.5	32.8
K09	100	15.6	13.7	1 292.0	1 347.2	94.3	98.4	95.8	25.6	33
K10	99.5	15.8	14.7	1 348.4	1 391.1	92	95	96.7	24.7	33.2
Wuyujing 3	98.6	15.6	13.6	1 258.5	1 325.4	93.6	98.6	95	25.9	32.8

Line/Variety	PH (cm)	PL (cm)	PP	FGPL	SPL	FGPA	SPA	SSR (%)	TGW (g)	GWP (g)
K01	97.9	15.7	14.3	1 397.9	1 452.2	98.6	102.6	96.3	25	33.6
K02	99.4	15	14.6	1 421.1	1 475.3	97.2	101	96.2	24.7	32.7
K03	99.1	15.4	14.3	1 289.6	1 354.7	90.8	95.5	95.2	24.7	31.2
K04	99.8	15.9	14.9	1 336.7	1 383.6	89.3	92.6	96.5	24.9	32.3
K05	99.7	15.5	13.4	1 249.0	1 291.6	93.6	96.8	96.7	25.9	32.1
K06	101.5	15.3	13.9	1 324.7	1 372.3	95.3	98.8	96.4	25.5	33.7
K07	100.2	15.9	13.4	1 288.7	1 329.5	96.3	99.4	96.9	25.1	32.2
K08	100.2	15.1	14	1 322.9	1 390.8	94.7	99.6	95.1	25.5	32.8
K09	100	15.6	13.7	1 292.0	1 347.2	94.3	98.4	95.8	25.6	33
K10	99.5	15.8	14.7	1 348.4	1 391.1	92	95	96.7	24.7	33.2
Wuyujing 3	98.6	15.6	13.6	1 258.5	1 325.4	93.6	98.6	95	25.9	32.8

Line/Variety	Lishui, Jiangsu Province		Taizhou, Jiangsu Province		Average
Line/Variety	Yield (kg/hm²)	Order	Yield (kg/hm²)	Order	Yield (kg/hm²)	Order
K01	7 543.5	5	10 680.0	4	9 111.8	3
K02	7 362.0	10	10 740.0	3	9 051.0	5
K03	7 335.0	11	10 132.5	10	8 733.8	11
K04	7 422.0	9	10 822.5	2	9 122.3	2
K05	7 485.0	6	10 035.0	11	8 760.0	10
K06	7 755.0	2	10 732.5	1	9 243.8	1
K07	7 887.0	1	10 155.0	9	9 021.0	6
K08	7 618.5	4	10 305.0	7	8 961.8	8
K09	7 740.0	3	10 432.5	6	9 086.3	4
K10	7 437.0	7	10 545.0	5	8 991.0	7
Wuyujing 3	7 429.5	8	10 257.0	8	8 843.3	9

Genetic Improvement of Japonica Rice Variety Wuyujing 3 for Stripe Disease Resistance and Eating Quality by Pyramiding Stv-bⁱ and Wx-mq

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References 22

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Line/Variety	Milling quality			Appearance quality				Eating quality
Line/Variety	Brown rice rate (%)	Milled rice rate (%)	Head milled rice rate (%)	Grain length	Length/width ratio	Chalky grain rate (%)	Degree of chalkiness (%)	Gel consistency	Alkali spreading value	Amylose content (%)
K01	79.5	71.8	68.5	4.72	1.65	8.5**	1.2**	80.8**	5.8	8.2**
K02	78.7	70.2	65.8	4.46	1.65	24.2**	4.2**	81.2**	5.8	8.2**
K03	78.7	70.9	68	4.53	1.68	13.7**	2.9**	79.7**	5.8	7.5**
K04	79.8	71.9	68.1	4.53	1.66	14.7**	2.9**	80.1**	5.7	7.5**
K05	80.3	72.5	68.6	4.98	1.63	23.9**	4.0**	81.1**	5.8	7.6**
K06	80.3	71.8	68.7	4.95	1.63	14.1**	2.1**	81.2**	5.8	7.6**
K07	79.2	71.2	68.5	4.74	1.69	13.7**	2.9**	81.5**	5.8	8.1**
K08	79.5	71.2	68.2	4.93	1.66	12.2**	2.2**	81.7**	5.7	8.7**
K09	79.7	72.4	69.2	4.93	1.67	18.0**	4.0**	81.7**	5.7	8.9**
K10	78.7	71.6	69.1	4.7	1.66	21.8**	4.5**	82.2**	5.8	8.9**
Wuyujing 3	78.8	70.1	67.1	4.99	1.61	45.2	9.2	75.8	5.8	14.2

Line/Variety	Odor	Appearance	Palatability	Fragrance	Comprehensive score	Taste value
K01	0.1	0.8	1.40**	0.90*	1.50**	0.96**
K02	0.2	1.10*	1.30*	1.20**	1.50**	1.13**
K03	0.1	0.4	0.7	0.5	0.8	0.51
K04	0.1	0.90*	0.9	0.7	0.9	0.74
K05	0.1	0.7	0.6	0.6	0.7	0.58
K06	0	0.5	1	1.00*	1.38*	0.84*
K07	0.1	1.20**	1.70**	1.60**	1.90**	1.42**
K08	0.1	1.10*	1.40**	0.90*	1.50**	1.02**
K09	0	0.90*	1.30*	1.30**	1.40*	1.10**
K10	0.2	0.90*	1.30*	1.20**	1.20*	1.16**
Wuyujing 3	0	0	0	0	0	0

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