Rice Science ›› 2015, Vol. 22 ›› Issue (6): 264-274.DOI: 10.1016/S1672-6308(14)60306-1

• Orginal Article • Previous Articles     Next Articles

Genetic Dissection of Low Phosphorus Tolerance Related Traits Using Selected Introgression Lines in Rice

Chao Xiang1, Jie Ren1,2,3, Xiu-qin Zhao1, Zai-song Ding1, Jing Zhang1,2, Chao Wang1,2, Jun-wei Zhang1,2, Augustino Joseph Charles1, Qiang Zhang1, Yun-long Pang1, Yong-ming Gao1(), Ying-yao Shi2()   

  1. 1Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    2College of Agronomy, Anhui Agricultural University, Hefei 230036, China
    3Agricultural Bureau of Deqing, Huzhou 313000, China
    #These authors contribute equally to this work
  • Received:2015-04-06 Accepted:2015-05-08 Online:2015-06-06 Published:2015-09-15

Abstract:

To dissect the genetic basis of low phosphorus tolerance (LPT), 114 BC2F4 introgression lines (ILs) were developed from Shuhui 527 and Minghui 86 (recurrent parents), and Yetuozai (donor parent). The progenies were tested for 11 quantitative traits under three treatments including normal fertilization in normal soil (as control), normal fertilization in barren soil and low phosphorus stress in barren soil in Langfang, Hebei Province, China. Moreover, the ILs were investigated at the seedling stage using nutrient solution culture method in greenhouse in Beijing, China. A total of 49 main-effect quantitative trait loci (QTLs) underlying yield related traits were identified in Langfang, and their contributions to phenotypic variations ranged from 6.7% to 16.5%. Among them, 25 (51.0%) QTLs had favorable alleles from donor parent. A total of 48 main-effect QTLs were identified for LPT-related traits in Beijing, and their contributions to phenotypic variations ranged from 7.7% to 16.6%. Among them, 21 (43.8%) QTLs had favorable alleles from donor parent. About 79.6% of the QTLs can be detected repeatedly under two or more treatments, especially QTLs associated with spikelet number per panicle, spikelet fertility and 1000-grain weight, displaying consistent phenotypic effects. Among all the detected QTLs, eight QTLs were simultaneously identified under low phosphorus stress across two environments. These results can provide useful information for the genetic dissection of LPT in rice.

Key words: rice, phosphorus tolerance, yield, introgression line, quantitative trait locus