Screening of Rice Genes Interacting with p5b of Rice Black-Streaked Dwarf Virus

doi:10.1016/S1672-6308(13)60128-6

RICE SCIENCE

• Research Paper • Previous Articles Next Articles

Screening of Rice Genes Interacting with p5b of Rice Black-Streaked Dwarf Virus

1State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control / Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture / Zhejiang Provincial Key Laboratory of Plant Virology / Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China

Revised:2012-12-31 Online:2013-05-28 Published:2013-03-27
Contact: ZHANG Heng-mu (zhhengmu@tsinghua.org.cn) CHEN Jian-ping (jpchen2001@yahoo.com.cn)
Supported by:
This work was funded by the National Science and Technology Support Program of China (Grant No. 2012BAD19B03), the National High Technology Research and Development Program of China (Grant No. 2007AA10Z414), the National Basic Research Program of China (Grant No. 2010CB126203), the International Science and Technology Cooperation Project (Grant No. 2007DFB30350), the Special Fund for Agro-scientific Research in the Public Interest of China (Grant No. 201003031), the Zhejiang Provincial Science and Technology Project, China (Grant No. 2010C12027), and the Zhejiang Provincial Foundation for Natural Science, China (Grant Nos. Z305165 and Y3090657).

Abstract

Abstract:

Rice black-streaked dwarf virus (RBSDV) is a recognized member of the genus Fijivirus, family Reoviridae. Its genome has ten double-stranded RNA (dsRNA) segments (S1–S10), in which the fifth genome segment (S5) contains two open reading frames (ORFs) with a partially overlapping region. The second ORF of RBSDV S5 encodes a viral nonstructural protein named p5b with unknown function. To reveal the function of p5b, its gene was ligated into the bait plasmid pGBKT7 and an expression library containing rice cDNAs was constructed using plasmid pGADT7 for yeast two-hybrid assay. The bait protein p5b was detected in yeast by western blot, and the result of an auto-activation test showed that p5b could not autonomously activate the expression of reporter genes in yeast. Then the bait protein p5b was used for screening the cDNA expression libraries of rice. Gene fragments of some pivotal enzymes involved in photosynthesis, respiration and other important metabolic processes, were identified to interact with p5b in yeast, suggesting that these interactions may play roles in symptom development in infected plants.

Key words: rice black-streaked dwarf virus, interaction protein, yeast two-hybrid

LU Ying1, 2, YANG Jian 1, ZHANG Heng-mu1, CHEN Jian-ping1. Screening of Rice Genes Interacting with p5b of Rice Black-Streaked Dwarf Virus[J]. RICE SCIENCE, DOI: 10.1016/S1672-6308(13)60128-6 .

References

Bykova N V, Keerberg O, P?rnik T, Bauwe H, Gardestr?m P. 2005. Interaction between photorespiration and respiration in transgenic potato plants with antisense reduction in glycine decarboxylase. Planta, 222: 130–140.
Chen S X. 1996. Occurrence situation and research progress of viral disease on rice plants. J Zhejiang Agric Sci, (1): 41–42. (in Chinese)
Fauquet C M, Mayo M A, Maniloff J, Desselberger U, Ball L A. 2005. Virus taxonomy: Classification and nomenclature of viruses. In: Eighth Report of the International Committee on the Taxonomy of Viruses. San Diego: Elsevier Academic Press: 534–542.
Fields S, Song O. 1989. A novel genetic system to detect protein-protein interactions. Nature, 340: 245–246.
Green P J. 1994. The ribonucleases of higher plants. Annu Rev Plant Physiol Plant Mol Biol, 45: 421–445.
Hibino H. 1996. Biology and epidemiology of rice viruses. Annu Rev Phytopath, 34: 249–274.
Milne R G, del Vas M, Harding R M, Marzachì R, Mertens P P C. Genus Fijirirus. In: Fauquet C M, Mayo M A, Maniloff J, Desselberger U, Ball L A. Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. 2005. San Diego: Elsevier Academic Press: 534–542.
Isogai M, Uyeda I, Lee B C. 1998. Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10. J Gen Virol, 79(6): 1487–1494.
Jia Y G, Zhang L J, Liu C, Song C, Cui Z H, Zhu Y S. 2009. Pyruvate orthophosphate dikinases and their gene structure. Plant Physiol Comm, 45(3): 305–311.
Kang H G, Park S, Matsuoka M, An G. 2005. White-core endosperm floury endosperm-4 in rice is generated by knockout mutations in the C4-type pyruvate orthophosphate dikinase gene (OsPPDKB). Plant J, 42: 901–911.
Krüger J, Thomas C M, Golstein C, Dixon M S, Smoker M, Tang S, Mulder L, Jones J D. 2002. A tomato cysteine protease required for Cf-2-dependent disease resistance and suppression of autonecrosis. Science, 296: 744–747.
Lu M F, Yang J, Zhang H M, Chen J P. 2012. Detection of two nonstructural proteins encoded by genome segment S7 of rice black-streaked dwarf virus in infected rice plants. Chin J Rice Sci, 25(1): 9–15. (in Chinese with English abstract)
Mohan S, Ma P W, Pechan T, Bassford E R, Williams W P, Luthe D S. 2005. Degradation of the S. frugiperda peritrophic matrix by an inducible maize cysteine protease. J Insect Physiol, 52: 21–28.
Moons A, Valcke R, Van Montagu M. 1998. Low-oxygen stress and water deficit induce cytosolic pyruvate orthophosphate dikinase (PPDK) expression in roots of rice, a C3 plant. Plant J, 15(1): 89–98.
Oliver D J, Raman R. 1995. Glycine decarboxylase: Protein chemistry and molecular biology of the major protein in leaf mitochondria. J Bioenerg Biomembr, 27(4): 407–414.
Ristic Z, Momcilovic I, Bukovnik U, Prasad P V, Fu J, Deridder B P, Elthon T E, Mladenov N. 2009. Rubisco activase and wheat productivity under heat-stress conditions. J Exp Bot, 60: 4003–4014.
Ruan Y L, Chen S X, Lin R F, Jiang W L, Jin D D. 1984. Study on rice black-streaked dwarf disease. J Zhejinag Agric Sci, (4): 185–192. (in Chinese)
Sambrook J, Russell D. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. New York: Cold Spring Harbory Laboratory Press.
Shikata E, Kitagawa Y. 1977. Rice black-streaked dwarf virus: Its properties, morphology, and intracellular localization. Virology, 77: 826–842.
Uyeda I, Lee B C, Ando Y, Suga H, He Y K, Isogai M. 1998. Reovirus isolation and RNA extraction. Methods Mol Biol, 81: 65–75.
Willekens H, Chamnongpol S, Davey M, Schraudner M, Langebartels C, Van Montagu M, Inzé D, Van Camp W. 1997. Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. EMBO J, 16: 4806–4816.
Yang Q, Reinhard K, Schiltz E, Matern U. 1997. Characterization and heterologous expression of hydroxylcinnamoyl/benzoyl-CoA: Anthranilate N-hydroxycinnamoyl/benzoyltransferase from elicited cell cultures of carnation, Dianthus caryophyllus L. Plant Mol Biol, 35: 777–789.
Zhang H M. 2001. Molecular biology of rice black-streaked dwarf virus. [PhD dissertation]. Hangzhou: Zhejiang University. (in Chinese with English abstract)
Zhang H M, Chen J P, Lei J L, Adams M J. 2001a. Sequence analysis shows a dwarf disease on rice, wheat and maize in China is caused by rice black-streaked dwarf virus. Eur J Plant Pathol, 107(5): 563–567.
Zhang H M, Chen J P, Adams M J. 2001b. Molecular characterisation of segments 1 to 6 of rice black-streaked dwarf virus from China provides the complete genome. Arch Virol, 146: 2331–2339.
Zhou F, Wu G, Deng W L, Pu Y Y, Wei C H, Li Y. 2007. Interaction of rice dwarf virus outer capsid P8 protein with rice glycolate oxidase mediates relocalization of P8. FEBS Lett, 581(1): 34–40.
Zhu S F, Gao F, Cao X S, Chen M, Ye G Y, Wei C H, Li Y. 2005. The rice dwarf virus P2 protein interacts with ent-kaurene oxidases in vivo, leading to reduced biosynthesis of gibberellins and rice dwarf symptoms. Plant Physiol, 139(4): 1935–1945.

Screening of Rice Genes Interacting with p5b of Rice Black-Streaked Dwarf Virus

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics