RICE SCIENCE ›› 2011, Vol. 18 ›› Issue (2): 102-109.

• Research Paper • Previous Articles     Next Articles

Transferring Translucent Endosperm Mutant Gene Wx-mq and Rice Stripe Disease Resistance Gene Stv-bi by Marker-Assisted Selection in Rice (Oryza sativa)

YAO Shu1, 2, CHEN Tao1, ZHANG Ya-dong1, ZHU Zhen1, ZHAO Ling1, ZHAO Qing-yong1, ZHOU Li-hui1, WANG Cai-lin1   

  1. 1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice R & D Center, Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China; 2College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China
  • Online:2011-06-28 Published:2011-08-22
  • Contact: WANG Cai-lin
  • Supported by:

    This work was supported by the Key Program of the Development of Variety of Genetically Modified Organisms (Grant Nos. 2009ZX08001-019B and 2008ZX08001-006), the Special Program for Rice Scientific Research of Ministry of Agriculture (Grant No. nyhyzx 07-001-006), the Key Support Program of Science and Technology of Jiangsu Province (Grant No. BE2008354), and the Self-directed Innovation Fund of Agricultural Science and Technology in Jiangsu Province, China (Grant No. CX [09] 634).

Abstract: A high-yielding japonica rice variety, Wuyunjing 7, bred in Jiangsu Province, China as a female parent was crossed with a Japanese rice variety Kantou 194, which carries a rice stripe disease resistance gene Stv-bi and a translucent endosperm mutant gene Wx-mq. From F2 generations, a sequence characterized amplified region (SCAR) marker tightly linked with Stv-bi and a cleaved amplified polymorphic sequence (CAPS) marker for Wx-mq were used for marker-assisted selection. Finally, a new japonica rice line, Ning 9108, with excellent agronomic traits was obtained by multi-generational selection on stripe disease resistance and endosperm appearance. The utilization of the markers from genes related to rice quality and disease resistance was helpful not only for establishing a marker-assisted selection system of high-quality and disease resistance for rice but also for providing important intermediate materials and rapid selection method for good quality, disease resistance and high yield in rice breeding.

Key words: rice, translucent endosperm mutant gene, rice stripe disease resistance gene, marker-assisted selection