Development of Simple Functional Markers for Low Glutelin Content Gene 1 (Lgc1) in Rice (Oryza sativa)

doi:10.1016/S1672-6308(09)60014-7

RICE SCIENCE ›› 2010, Vol. 17 ›› Issue (3): 173-178 .DOI: 10.1016/S1672-6308(09)60014-7

• Research Paper • Previous Articles Next Articles

Development of Simple Functional Markers for Low Glutelin Content Gene 1 (Lgc1) in Rice (Oryza sativa)

CHEN Tao^#, TIAN Meng-xiang^#, ZHANG Ya-dong, ZHU Zhen, ZHAO Ling, ZHAO Qing-yong, LIN Jing, ZHOU Li-hui, WANG Cai-lin

Jiangsu High Quality Rice Research and Development Center, Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; ^# These authors contributed equally to this paper

Received:2010-02-03 Revised:1900-01-01 Online:2010-09-28 Published:2010-09-28
Contact: WANG Cai-lin
Supported by:
the National Transgenic Crops Program, China (Grant No. 2008ZX08001-006), the Research Funds for Public Benefit in Ministry of Agriculture, China (Grant No. 200803056), the Key Support Program of Jiangsu Science and Technology, China (Grant No. BE2008354) and the Self-directed Innovation Fund of Agricultural Science and Technology in Jiangsu Province, China (Grant No. CX [08]603).

Abstract

Abstract: Rice with low glutelin content is suitable as functional food for patients affected by kidney failure. Low glutelin- content gene Lgc1 in rice has a 3.5-kb deletion between two highly similar glutelin genes GluB4 and GluB5, which locates on the short arm of chromosome 2. To improve the selection efficiency in low glutelin-content rice breeding, two molecular markers designated as InDel-Lgc1-1 and InDel-Lgc1-2 were developed to detect the low glutelin-content gene Lgc1. A double PCR detection indicated that combined use of the two markers could easily distinguish the genotypes of Lgc1 from different rice varieties. Therefore, as a simple and low-cost technique, the molecular marker could be widely used to identify different varieties with Lgc1 gene and applied in marker-assisted selection of low glutelin-content rice.

Key words: Oryza sativa, low glutelin-content gene, functional marker, double polymerase chain reaction

CHEN Tao, TIAN Meng-xiang, ZHANG Ya-dong, ZHU Zhen, ZHAO Ling, ZHAO Qing-yong, LIN Jing, ZHOU Li-hui, WANG Cai-lin. Development of Simple Functional Markers for Low Glutelin Content Gene 1 (Lgc1) in Rice (Oryza sativa)[J]. RICE SCIENCE, 2010, 17(3): 173-178 .

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Development of Simple Functional Markers for Low Glutelin Content Gene 1 (Lgc1) in Rice (Oryza sativa)

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